Purification of PRL receptors from toad kidney: comparisons with rabbit mammary PRL receptors

Détails

ID Serval
serval:BIB_C025FE589B89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Purification of PRL receptors from toad kidney: comparisons with rabbit mammary PRL receptors
Périodique
American Journal of Physiology
Auteur(s)
Dunand  M., Kraehenbuhl  J. P., Rossier  B. C., Aubert  M. L.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
03/1988
Volume
254
Numéro
3 Pt 1
Pages
C372-82
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
The binding characteristics of the prolactin (PRL) receptors present in toad (Bufo marinus) kidneys were investigated and compared to those of PRL receptors present in rabbit mammary glands. The molecular characteristics of the Triton X-100 solubilized renal and mammary PRL receptors were assessed by gel filtration and by migration analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after affinity labeling of the binding sites with 125I-human growth hormone. Similar results were obtained for both receptors. Partial purification of the toad PRL receptor could be achieved by affinity chromatography. The molecular weight of this purified receptor could be determined by analysis on SDS-PAGE. With the use of a polyclonal antiserum raised against a purified preparation of rabbit mammary PRL receptor, one or several antigenic epitope(s) could be identified on the core of the toad renal PRL receptor. In conclusion, although the structure and the biological role(s) of PRL have substantially changed during evolution, the receptor for this hormone has retained many of its structural features as could be assessed between an amphibian and a mammalian species on functionally different target tissues.
Mots-clé
Affinity Labels/metabolism Animals Bufo marinus Chromatography, Gel Immune Sera/immunology Kidney/*analysis/metabolism Mammary Glands, Animal/*analysis/metabolism Rabbits Receptors, Prolactin/immunology/*isolation & purification/metabolism Solubility
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
03/03/2018 21:05
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