Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens

Détails

ID Serval
serval:BIB_C016389267D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens
Périodique
Journal of Proteome Research
Auteur(s)
Asif  A. R., Oellerich  M., Amstrong  V. W., Riemenschneider  B., Monod  M., Reichard  U.
ISSN
1535-3893 (Print)
Statut éditorial
Publié
Date de publication
04/2006
Volume
5
Numéro
4
Pages
954-62
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
Aspergillus fumigatus is a mold causing most of the invasive fungal lung infections in the immunocompromised host. In addition, the species is the causative agent of certain allergic diseases. Both in invasive and in allergic diseases, the conidial surface mediates the first contact with the human immune system. Thus, conidial surface proteins may be reasonable vaccine candidates as well as important allergens. To broaden the list of those antigens, intact viable Aspergillus conidia were extracted with mild alkaline buffer at pH 8.5 in the presence of a 1,3-beta-glucanase. The proteome of this fraction was separated by two- dimensional gel electrophoresis (2-DE) and analyzed by liquid chromatography coupled with tandem mass spectrometry. Altogether 26 different A. fumigatus proteins were identified, twelve of which contain a signal for secretion. Among these were the known major conidial surface protein rodlet A, one acid protease PEP2, one lipase, a putative disulfide isomerase and a putative fructose-1,6-biphosphatase. The known allergen Aspf 3 was identified among the proteins without a signal for secretion. On the basis of the recently annotated A. fumigatus genome (Nature 2005, 438, 1151-1156), proteome analysis is now a powerful tool to confirm expression of hypothetical proteins and, thereby to identify additional vaccine candidates and possible new allergens of this important fungal pathogen.
Mots-clé
*Allergens Antigens, Fungal/genetics/immunology Aspergillus fumigatus/genetics/growth & development/*immunology/physiology Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Formic Acids/chemistry *Fungal Vaccines Isoelectric Focusing Isoelectric Point Mass Spectrometry Membrane Proteins/*analysis/immunology Molecular Weight Peptide Mapping Proteome/*analysis Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spores, Fungal/*chemistry Trypsin/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 17:47
Dernière modification de la notice
03/03/2018 21:05
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