An essential role for the stalk region of CD8 beta in the coreceptor function of CD8.

Détails

ID Serval
serval:BIB_C015C2B5A4D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An essential role for the stalk region of CD8 beta in the coreceptor function of CD8.
Périodique
Journal of immunology
Auteur(s)
Rettig L., McNeill L., Sarner N., Guillaume P., Luescher I., Tolaini M., Kioussis D., Zamoyska R.
ISSN
1550-6606[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
182
Numéro
1
Pages
121-129
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The CD8alphabeta heterodimer is integral to the selection of the class I-restricted lineage in the thymus; however, the contribution of the CD8beta chain to coreceptor function is poorly understood. To understand whether the CD8beta membrane proximal stalk region played a role in coreceptor function, we substituted it with the corresponding sequence from the CD8alpha polypeptide and expressed the hybrid molecule in transgenic mice in place of endogenous CD8beta. Although the stalk-swapped CD8beta was expressed on the cell surface as a disulfide-bonded heterodimer at equivalent levels of expression to an endogenous CD8beta molecule, it failed to restore selection of CD8(+) class I MHC-restricted T cells and it altered the response of peripheral T cells. Thus, the stalk region of the CD8beta polypeptide has an essential role in ensuring functionality of the CD8alphabeta heterodimer and its replacement compromises the interaction of CD8 with peptide-MHC complexes.
Mots-clé
Amino Acid Sequence, Animals, Antigens, CD8/biosynthesis, Antigens, CD8/genetics, CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Cell Differentiation/genetics, Cell Differentiation/immunology, Cells, Cultured, Dimerization, Gene Deletion, Gene Fusion/immunology, Histocompatibility Antigens Class I/genetics, Histocompatibility Antigens Class I/immunology, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Knockout, Mice, Transgenic, Molecular Sequence Data, Protein Structure, Tertiary/genetics, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/physiology, Transgenes/immunology
Pubmed
Web of science
Création de la notice
15/01/2010 16:28
Dernière modification de la notice
03/03/2018 21:05
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