Immunogenicity of multiple antigen peptides containing Plasmodium vivax CS epitopes in BALB/c mice

Details

Serval ID
serval:BIB_BF74D42C981E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunogenicity of multiple antigen peptides containing Plasmodium vivax CS epitopes in BALB/c mice
Journal
Memorias do Instituto Oswaldo Cruz
Author(s)
Herrera  M. A., de Plata  C., Gonzalez  J. M., Corradin  G., Herrera  S.
ISSN
0074-0276 (Print)
Publication state
Published
Issued date
1994
Volume
89 Suppl 2
Pages
71-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
Multiple antigen peptide systems (MAPs) allow the incorporation of various epitopes in to a single synthetic peptide immunogen. We have characterized the immune response of BALB/c mice to a series of MAPs assembled with different B and T cell epitopes derived from the Plasmodium vivax circumsporozoite (CS) protein. A B-cell epitope from the central repeat domain and two T-cell epitopes from the amino and carboxyl flanking regions were used to assembled eight different MAPs. An additional universal T cell epitope (ptt-30) from tetanus toxin protein was included. Immunogenicity in terms of antibody responses and in vitro T lymphocyte proliferation was evaluated. MAPs containing B and T cell epitopes induced high titers of anti-peptides antibodies, which recognized the native protein on sporozoites as determined by IFAT. The antibody specificity was also determined by a competitive inhibition assay with different MAPs. A MAP containing the B cell epitope (p11) and the universal epitope ptt-30 together with another composed of p11 and the promiscuous T cell epitope (p25) proved to be the most immunogenic. The strong antibody response and specificity for the cognate protein indicates that further studies designed to assess the potential of these proteins as human malaria vaccine candidates are warranted.
Keywords
Animals Enzyme-Linked Immunosorbent Assay Epitopes/immunology Malaria/*immunology Mice Mice, Inbred BALB C Peptides/*immunology Plasmodium vivax/*immunology Vaccines, Synthetic/immunology
Pubmed
Web of science
Create date
24/01/2008 14:54
Last modification date
20/08/2019 15:33
Usage data