One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.

Détails

Ressource 1Télécharger: 28525571_BIB_BF5DFD01F732.pdf (1905.01 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_BF5DFD01F732
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.
Périodique
Genome biology and evolution
Auteur(s)
Mueller L., Bertelli C., Pillonel T., Salamin N., Greub G.
ISSN
1759-6653 (Electronic)
ISSN-L
1759-6653
Statut éditorial
Publié
Date de publication
01/06/2017
Peer-reviewed
Oui
Volume
9
Numéro
6
Pages
1432-1449
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Amoeba-resisting microorganisms raised a great interest during the last decade. Among them, some large DNA viruses present huge genomes up to 2.5 Mb long, exceeding the size of small bacterial genomes. The rate of genome evolution in terms of mutation, deletion, and gene acquisition in these genomes is yet unknown. Given the suspected high plasticity of viral genomes, the microevolution of the 346 kb genome of Lausannevirus, a member of Megavirales, was studied. Hence, Lausannevirus was co-cultured within the amoeba Acanthamoeba castellanii over one year. Despite a low number of mutations, the virus showed a genome reduction of 3.7% after 12 months. Lausannevirus genome evolution in sympatric conditions was investigated by its co-culture with Estrella lausannensis, an obligate intracellular bacterium, in the amoeba A. castellanii during one year. Cultures were split every 3 months. Genome sequencing revealed that in these conditions both, Lausannevirus and E. lausannensis, show stable genome, presenting no major rearrangement. In fact, after one year they acquired from 2 to 7 and from 4 to 10 mutations per culture for Lausannevirus and E. lausannensis, respectively. Interestingly, different mutations in the endonuclease encoding genes of Lausannevirus were observed in different subcultures, highlighting the importance of this gene product in the replication of Lausannevirus. Conversely, mutations in E. lausannensis were mainly located in a gene encoding for a phosphoenolpyruvate-protein phosphotransferase (PtsI), implicated in sugar metabolism. Moreover, in our conditions and with our analyses we detected no horizontal gene transfer during one year of co-culture.

Mots-clé
Chlamydiales, Lausannevirus, Marseilleviridae, Megavirales, experimental evolution, single nucleotide polymorphisms (SNPs)
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/05/2017 17:33
Dernière modification de la notice
09/05/2019 0:39
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