Protein kinases expressed in aggregating brain cell cultures during myelination.

Details

Serval ID
serval:BIB_BED7C1ADB01C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Protein kinases expressed in aggregating brain cell cultures during myelination.
Journal
Schweizer Archiv für Neurologie und Psychiatrie
Author(s)
Miescher G.C., Taylor V., Kappos L., Matthieu J.M., Honegger P.
ISSN
0258-7661 (Print)
ISSN-L
0258-7661
Publication state
Published
Issued date
1993
Volume
144
Number
3
Pages
217-221
Language
english
Abstract
Serum-free aggregating rat brain cell cultures provide sufficient cell surface and paracrine interactions between neurons and glial cells for compact myelination. We are interested in the part played in these signalling pathways by protein kinases and have used a PCR cDNA cloning approach to catalogue the protein kinase genes expressed by these cultures. 8 transmembrane protein kinases were identified: IGF1-R, trk B, bFGF-R, c-met, Tyro2, Tyro1, Tyro4 and a novel eck-related gene. The first 4 are receptors for ligands with known trophic functions. Tyro2 is a novel gene related to the EGF-R. The latter 3 belong to the eck gene family of more than 8 highly related putative receptors for, as yet, unknown ligands. 8 cDNAs for intracellular protein kinases were also isolated including 3 novel genes. Ongoing studies are investigating whether these proteins contribute to myelination and/or could be used as therapeutic targets in demyelinating diseases.
Keywords
Animals, Brain/cytology, Cell Aggregation/genetics, Cell Differentiation/genetics, Culture Techniques, DNA/genetics, Gene Expression Regulation/physiology, Myelin Proteins/genetics, Polymerase Chain Reaction, Protein Kinases/genetics, Rats
Pubmed
Create date
24/01/2008 13:11
Last modification date
20/08/2019 15:33
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