New microfluidic-based sampling procedure for overcoming the hematocrit problem associated with dried blood spot analysis.

Détails

ID Serval
serval:BIB_BED19111B5DF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
New microfluidic-based sampling procedure for overcoming the hematocrit problem associated with dried blood spot analysis.
Périodique
Analytical Chemistry
Auteur(s)
Leuthold L.A., Heudi O., Déglon J., Raccuglia M., Augsburger M., Picard F., Kretz O., Thomas A.
ISSN
1520-6882 (Electronic)
ISSN-L
0003-2700
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
87
Numéro
4
Pages
2068-2071
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Hematocrit (Hct) is one of the most critical issues associated with the bioanalytical methods used for dried blood spot (DBS) sample analysis. Because Hct determines the viscosity of blood, it may affect the spreading of blood onto the filter paper. Hence, accurate quantitative data can only be obtained if the size of the paper filter extracted contains a fixed blood volume. We describe for the first time a microfluidic-based sampling procedure to enable accurate blood volume collection on commercially available DBS cards. The system allows the collection of a controlled volume of blood (e.g., 5 or 10 μL) within several seconds. Reproducibility of the sampling volume was examined in vivo on capillary blood by quantifying caffeine and paraxanthine on 5 different extracted DBS spots at two different time points and in vitro with a test compound, Mavoglurant, on 10 different spots at two Hct levels. Entire spots were extracted. In addition, the accuracy and precision (n = 3) data for the Mavoglurant quantitation in blood with Hct levels between 26% and 62% were evaluated. The interspot precision data were below 9.0%, which was equivalent to that of a manually spotted volume with a pipet. No Hct effect was observed in the quantitative results obtained for Hct levels from 26% to 62%. These data indicate that our microfluidic-based sampling procedure is accurate and precise and that the analysis of Mavoglurant is not affected by the Hct values. This provides a simple procedure for DBS sampling with a fixed volume of capillary blood, which could eliminate the recurrent Hct issue linked to DBS sample analysis.
Pubmed
Web of science
Création de la notice
26/01/2015 8:25
Dernière modification de la notice
20/08/2019 15:33
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