Soil tillage affects the community structure of mycorrhizal fungi in maize roots

Détails

ID Serval
serval:BIB_BDC757774227
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Soil tillage affects the community structure of mycorrhizal fungi in maize roots
Périodique
Ecological Applications
Auteur(s)
Jansa J., Mozafar A., Kuhn G., Anken T., Ruh R., Sanders I. R., Frossard E.
ISSN
1051-0761
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
13
Numéro
4
Pages
1164-1176
Langue
anglais
Résumé
In this study we tested whether communities of arbuscular mycorrhizal fungi (AMF) colonizing the roots of maize (Zea mays L.) were affected by soil tillage practices (plowing, chiseling, and no-till) in a long-term field experiment carried out in Tanikon (Switzerland). AMF were identified in the roots using specific polymerase chain reaction (PCR) markers that had been developed for the AMF previously isolated from the soils of the studied site. A nested PCR procedure with primers of increased specificity (eukaryotic, then, fungal, then AMF species or. species-grouop specific) was used. Sequencing of amplified DNA confirmed that the DNA obtained from the maize roots was of AMF origin. Presence of particular AMF species or species-group was scored as a presence of a DNA product after PCR with specific primers. We also used single-strand conformation polymorphism analysis (SSCP), of amplified DNA samples to-check if the amplification of the DNA from maize roots matched the expected profile for a particular AMF isolate with a given specific primer pair.
Presence of the genus Scutellospora, in maize roots was strongly reduced in plowed and chiseled soils. Fungi from the suborder Glomineae were more prevalent colonizers of maize roots growing in plowed soils, but were also present in the roots from other tillage treatments. These changes in community of AMF colonizing maize roots might be due to (1), the differences in tolerance to the tillage-induced disruption of the hyphae among the different AMF species, (2) changes in nutrient content of the soil, (3) changes in microbial activity, or (4) changes in weed populations in response to soil tillage. This is the first report on community composition of AMF in the roots of a field-grown crop plant (maize) as affected by soil tillage.
Mots-clé
arbuscular mycorrhiza, community structure, field experiment, maize, molecular identification, root colonization, soil tillage, specific primers
Web of science
Création de la notice
24/01/2008 18:38
Dernière modification de la notice
20/08/2019 16:32
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