Y chromosome microsatellite isolation from BAC clones in the greater white-toothed shrew (Crocidura russula)

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_BD9F535D925F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Y chromosome microsatellite isolation from BAC clones in the greater white-toothed shrew (Crocidura russula)
Périodique
Molecular Ecology Notes
Auteur(s)
Lawson Handley L.J., Perrin N.
ISSN
1471-8278
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
60
Numéro
1
Pages
276-279
Langue
anglais
Résumé
We constructed a microsatellite library from four Crocidura russula Y chromosome-specific bacterial artificial chromosome (BAC) clones. Only one of eight microsatellites was male-specific, despite genome walking to obtain more flanking sequence and testing of 93 primer combinations. Potential reasons for this low success are discussed. The male-specific locus, CRY3, was genotyped in 90 males, including C. russula from across the species range and two related species. The large difference in CRY3 allele size between eastern and western lineages supports earlier reports of high divergence between them. Despite polymorphism of CRY3 in Morocco, only one allele was found throughout the whole of Europe, consistent with previous studies that suggest recent colonization of Europe from a small number of Moroccan founders.
Mots-clé
bac library, chromosome, microsatellite library, retro-elements, shrews
Web of science
Création de la notice
19/11/2007 11:46
Dernière modification de la notice
03/03/2018 20:59
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