Imaging exocytosis and recycling of synaptic-like microvesicles in astrocytes.

Détails

ID Serval
serval:BIB_BD8497F4454A
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Imaging exocytosis and recycling of synaptic-like microvesicles in astrocytes.
Périodique
Cold Spring Harbor Protocols
Auteur(s)
Bezzi P., Volterra A.
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Statut éditorial
Publié
Date de publication
2014
Volume
2014
Numéro
5
Pages
537-543
Langue
anglais
Résumé
Optical imaging techniques are well suited for following the dynamics of physiological processes in living cells. Total internal reflection fluorescence (TIRF) microscopy based on evanescent wave illumination (EWi) allows spectacular, real-time visualization of individual vesicle movements, fusions, and retrievals at the cell surface (i.e., within 100 nm of the plasma membrane). TIRF microscopy is an ideal approach for studying the properties of exocytosis and recycling in cultured astrocytes, particularly because these cells have a rather flat surface and contain secretory vesicles with sparse distribution. Among all populations of secretory vesicles, we focus here on synaptic-like microvesicles (SLMVs). We illustrate how TIRF microscopy using EWi is useful to study exocytosis and recycling of SLMVs at the single-vesicle level and, when combined with epifluorescence illumination (EPIi), can provide detailed information on the kinetics of exocytosis, endocytosis, and re-acidification at the whole-cell level.
Mots-clé
Animals, Astrocytes/physiology, Exocytosis, Exosomes/metabolism, Humans, Microscopy, Fluorescence/methods, Optical Imaging/methods
Pubmed
Création de la notice
08/09/2014 14:25
Dernière modification de la notice
18/09/2018 10:57
Données d'usage