Imaging exocytosis and recycling of synaptic-like microvesicles in astrocytes.

Details

Serval ID
serval:BIB_BD8497F4454A
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Imaging exocytosis and recycling of synaptic-like microvesicles in astrocytes.
Journal
Cold Spring Harbor Protocols
Author(s)
Bezzi P., Volterra A.
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Publication state
Published
Issued date
2014
Volume
2014
Number
5
Pages
537-543
Language
english
Abstract
Optical imaging techniques are well suited for following the dynamics of physiological processes in living cells. Total internal reflection fluorescence (TIRF) microscopy based on evanescent wave illumination (EWi) allows spectacular, real-time visualization of individual vesicle movements, fusions, and retrievals at the cell surface (i.e., within 100 nm of the plasma membrane). TIRF microscopy is an ideal approach for studying the properties of exocytosis and recycling in cultured astrocytes, particularly because these cells have a rather flat surface and contain secretory vesicles with sparse distribution. Among all populations of secretory vesicles, we focus here on synaptic-like microvesicles (SLMVs). We illustrate how TIRF microscopy using EWi is useful to study exocytosis and recycling of SLMVs at the single-vesicle level and, when combined with epifluorescence illumination (EPIi), can provide detailed information on the kinetics of exocytosis, endocytosis, and re-acidification at the whole-cell level.
Keywords
Animals, Astrocytes/physiology, Exocytosis, Exosomes/metabolism, Humans, Microscopy, Fluorescence/methods, Optical Imaging/methods
Pubmed
Create date
08/09/2014 13:25
Last modification date
20/08/2019 15:31
Usage data