A recombinant Salmonella typhimurium vaccine strain is taken up and survives within murine Peyer's patch dendritic cells

Détails

ID Serval
serval:BIB_BD44C687363F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A recombinant Salmonella typhimurium vaccine strain is taken up and survives within murine Peyer's patch dendritic cells
Périodique
Cellular Microbiology
Auteur(s)
Hopkins  S. A., Niedergang  F., Corthesy-Theulaz  I. E., Kraehenbuhl  J. P.
ISSN
1462-5814 (Print)
Statut éditorial
Publié
Date de publication
02/2000
Volume
2
Numéro
1
Pages
59-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
The attenuated Salmonella typhimurium PhoPc strain is avirulent but immunogenic via the oral route in mice and is attenuated in survival in macrophage cell lines. In this study, the fate of PhoPc bacteria expressing green fluorescent protein was investigated in murine Peyer's patches. The survival of PhoPc was monitored after orogastric inoculation of BALB/c mice. Bacteria persisted for several weeks in the Peyer's patches and were also recovered from the mesenteric lymph nodes and spleen. Confocal microscopy analysis identified dendritic cells as the Peyer's patch cell type that internalized PhoPc expressing green fluorescent protein at early time points. In addition, live PhoPc were found in Peyer's patch dendritic cells and not in B cells 3 days after orogastric inoculation. Taken together, these results provide strong evidence that PhoPc is internalized and survives within Peyer's patch dendritic cells. As these cells are potent antigen-presenting cells, these data could explain the immunogenicity of S. typhimurium vaccine strains in vivo.
Mots-clé
Animals B-Lymphocytes/microbiology Colony Count, Microbial Dendritic Cells/*microbiology Gene Expression Green Fluorescent Proteins Indicators and Reagents/metabolism Intestinal Mucosa/microbiology Luminescent Proteins/genetics/metabolism Macrophages/microbiology Mice Mice, Inbred BALB C Peyer's Patches/*microbiology/ultrastructure Salmonella Vaccines/*immunology Salmonella typhimurium/genetics/growth & development/*immunology/ultrastructure Vaccines, Attenuated/immunology Vaccines, DNA/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
03/03/2018 20:58
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