MHC class I-restricted exogenous presentation of a synthetic 102-mer malaria vaccine polypeptide

Détails

ID Serval
serval:BIB_BD214ADB2463
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MHC class I-restricted exogenous presentation of a synthetic 102-mer malaria vaccine polypeptide
Périodique
European Journal of Immunology
Auteur(s)
Prato  S., Maxwell  T., Pinzon-Charry  A., Schmidt  C. W., Corradin  G., Lopez  J. A.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
03/2005
Volume
35
Numéro
3
Pages
681-9
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
The circumsporozoite (CS) is the most abundant surface protein of the Plasmodium sporozoite, and is also present early in the liver stage of the infection. Following successful protective experiments in mice and monkeys, the synthetic 102-mer malaria vaccine polypeptide representing the C-terminal region of the CS of Plasmodium falciparum was tested in a clinical trial with healthy human volunteers. This vaccine induced strong CD8(+), CD4(+) T lymphocyte and antibody responses specific for the immunizing peptide. CD8(+) T lymphocyte responses elicited in HLA-A*0201 volunteers recognized two well-defined cytotoxic T lymphocyte epitopes within the CS. Here, we show that both monocyte-derived dendritic cells (Mo-DC) and Epstein-Barr virus-transformed B-lymphoblastoid cells (LCL) can present a cytotoxic T lymphocyte epitope contained within the 102-mer synthetic peptide. Paraformaldehyde and low temperature inhibited presentation, indicating that cellular processing was required. Using specific inhibitors, we show that, in both cell types, processing requires the proteasome and the MHC class I pathway, while the endosomal compartment appears to be critical only for the presentation by Mo-DC. Antigen uptake is associated with actin polymerization in both cell types. These in vitro results demonstrate the likely pathway of antigen presentation achieved via vaccination with this synthetic peptide.
Mots-clé
Animals Antigen Presentation/*immunology B-Lymphocytes/immunology/virology Dendritic Cells/immunology Epitopes, T-Lymphocyte/immunology Flow Cytometry Herpesvirus 4, Human Histocompatibility Antigens Class I/*immunology Humans Malaria Vaccines/*immunology Peptides/*immunology Plasmodium falciparum/*immunology Protozoan Proteins/immunology Vaccines, Synthetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
20/08/2019 16:31
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