Lipopolysaccharide exposure modifies high tidal volume ventilation-induced proinflammatory mediator expression in newborn rat lungs

Détails

ID Serval
serval:BIB_BCFFA22CC3B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lipopolysaccharide exposure modifies high tidal volume ventilation-induced proinflammatory mediator expression in newborn rat lungs
Périodique
Pediatric Research
Auteur(s)
Roth-Kleiner  M., Ridsdale  R., Cao  L., Kuliszewski  M., Tseu  I., McKerlie  C., Post  M.
ISSN
0031-3998
Statut éditorial
Publié
Date de publication
02/2007
Peer-reviewed
Oui
Volume
61
Numéro
2
Pages
191-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
Infection/inflammation and mechanical ventilation have both independently been shown to increase cytokine/chemokine levels in lung tissue and blood samples of premature patients. Little is known about the combined effect of systemic inflammation and mechanical ventilation on cytokine expression in the lung. We tested whether pre-existing inflammation induced by lipopolysaccharide (LPS) exposure would modify cytokine/chemokine response in newborn rat lungs to high tidal volume ventilation (HTVV). Newborn rats were randomly assigned to four groups: groups I and II (saline); groups III and IV: 3 mg/kg LPS. Groups II and IV were 24h later subjected to 3h of ventilation with a tidal volume of 25 mL/kg. HTVV alone increased IL-1beta, IL-6 and the chemokine (C-X-C motif) ligand 2 (CXCL2) mRNA expression. Although the cytokine response to LPS alone had disappeared after 24 h, the combination of LPS pretreatment and HTVV significantly increased the expression of IL-6 and IL-1beta mRNA when compared with HTVV alone. TNF-alpha expression was increased neither by HTVV alone nor in combination with LPS. IL-6 protein content in bronchoalveolar lavage increased due to the combined treatment. Thus, a subtle pre-existing inflammation combined with HTVV amplifies the proinflammatory cytokine/chemokine expression in the newborn rat lung compared with HTVV alone.
Mots-clé
Animals Animals, Newborn Bronchoalveolar Lavage Fluid/chemistry Cytokines/genetics/*metabolism Lipopolysaccharides/*pharmacology Lung/drug effects/*growth & development/*immunology Pneumonia/etiology/immunology RNA, Messenger/metabolism Rats Rats, Wistar *Respiration, Artificial Tidal Volume Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 21:41
Dernière modification de la notice
09/05/2019 0:31
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