The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.

Détails

ID Serval
serval:BIB_BCED8EF9F2F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
Périodique
EMBO Journal
Auteur(s)
Le Hir H., Gatfield D., Izaurralde E., Moore M.J.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2001
Volume
20
Numéro
17
Pages
4987-4997
Langue
anglais
Résumé
We recently reported that spliceosomes alter messenger ribonucleoprotein particle (mRNP) composition by depositing several proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. When assembled in vitro, this so-called 'exon-exon junction complex' (EJC) contains at least five proteins: SRm160, DEK, RNPS1, Y14 and REF. To better investigate its functional attributes, we now describe a method for generating spliced mRNAs both in vitro and in vivo that either do or do not carry the EJC. Analysis of these mRNAs in Xenopus laevis oocytes revealed that this complex is the species responsible for enhancing nucleocytoplasmic export of spliced mRNAs. It does so by providing a strong binding site for the mRNA export factors REF and TAP/p15. Moreover, by serving as an anchoring point for the factors Upf2 and Upf3, the EJC provides a direct link between splicing and nonsense-mediated mRNA decay. Finally, we show that the composition of the EJC is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm.
Mots-clé
Animals, Antigens, Nuclear, Binding Sites, Cloning, Molecular, DNA-Binding Proteins/metabolism, Escherichia coli, Exons, Humans, Models, Genetic, Nuclear Matrix-Associated Proteins, Nuclear Proteins/metabolism, Oocytes/physiology, RNA Precursors/metabolism, RNA Splicing, RNA, Messenger/genetics, RNA, Messenger/metabolism, RNA-Binding Proteins/metabolism, Recombinant Proteins/metabolism, Ribonucleoproteins, Xenopus laevis
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/12/2012 12:21
Dernière modification de la notice
09/05/2019 0:31
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