Article: article from journal or magazin.
In vivo effects of a recombinant vaccinia virus expressing a mouse mammary tumor virus superantigen.
Journal of Virology
Early after infection, the mouse mammary tumor virus (MMTV) expresses a superantigen (SAg) at the surface of B lymphocytes. Interaction with the T-cell receptor Vbeta domain induces a polyclonal proliferative response of the SAg-reactive T cells. Stimulated T cells become anergic and are deleted from the T-cell repertoire. We have used a recombinant vaccinia virus encoding the MMTV(GR) SAg to dissect the effects of the retroviral SAg during an unrelated viral infection. Subcutaneous infection with this recombinant vaccinia virus induces a very rapid increase of Vbeta14 T cells in the draining lymph node. This stimulation does not require a large Plumber of infectious particles and is not strictly dependent on the expression of the major histocompatibility complex class II I-E molecule, as it is required after MMTV(GR) infection. In contrast to MMTV infection during which B cells are infected, we do not observe any clonal deletion of the reactive T cells following the initial stimulation phase. Our data show that contrary to the case with MMTV, macrophages but not B cells are the targets of infection by vaccinia virus in the lymph node, indicating the ability of these cells to present a retroviral SAg. The altered SAg expression in a different target cell observed during recombinant vaccinia virus infection therefore results in significant changes in the SAg response.
Animals, Antigens, Viral/biosynthesis, Antigens, Viral/immunology, Base Sequence, DNA Primers, Flow Cytometry, Gene Expression, Immunity, Cellular, Lymph Nodes/immunology, Lymph Nodes/virology, Mammary Tumor Virus, Mouse/immunology, Mammary Tumor Virus, Mouse/metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta/immunology, Recombination, Genetic, Superantigens/biosynthesis, Superantigens/immunology, T-Lymphocytes/immunology, T-Lymphocytes/virology, Time Factors, Vaccinia/immunology, Vaccinia virus/metabolism, Vaccinia virus/physiology, Viral Plaque Assay, Virus Replication
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