SREBP-1c expression in Schwann cells is affected by diabetes and nutritional status
Details
Serval ID
serval:BIB_BC5FF9C9E648
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SREBP-1c expression in Schwann cells is affected by diabetes and nutritional status
Journal
Molecular and Cellular Neurosciences
ISSN
1044-7431 (Print)
Publication state
Published
Issued date
08/2007
Volume
35
Number
4
Pages
525-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Our previous work demonstrated that the sterol response element binding proteins (SREBP)-1 and SREBP-2, which are the key regulators of storage lipid and cholesterol metabolism respectively, are highly expressed in Schwann cells of adult peripheral nerves. In order to evaluate the role of Schwann cell SREBPs in myelination and functioning of peripheral nerves we have determined their expression during development, after fasting and refeeding, and in a rodent model of diabetes. Our results show that SREBP-1c and SREBP-2, unlike SREBP-1a, are the major forms of SREBPs present in peripheral nerves. The expression profile of SREBP-2 follows the expression of genes involved in cholesterol biosynthesis, while SREBP-1c is co-expressed with genes involved in storage lipid metabolism. In addition, the expression of SREBP-1c in the endoneurial compartment of peripheral nerves depends on nutritional status and is disturbed in type 1 diabetes. In line with this, insulin elevates the expression of SREBP-1c in primary cultured Schwann cells by activating the SREBP-1c promoter. Taken together, these findings reveal that SREBP-1c expression in Schwann cells responds to metabolic stimuli including insulin and that this response is affected in type 1 diabetes mellitus. This suggests that disturbed SREBP-1c regulated lipid metabolism may contribute to the pathophysiology of diabetic peripheral neuropathy.
Keywords
Animals
Cells, Cultured
Diabetes Mellitus, Type 1/*metabolism
Fasting
Gene Expression Profiling
Gene Expression Regulation
Humans
Insulin/metabolism
Mice
*Nutritional Status
Oligonucleotide Array Sequence Analysis
Peripheral Nerves/metabolism
Promoter Regions (Genetics)
Protein Isoforms/genetics/*metabolism
Rats
Rats, Inbred Strains
Schwann Cells/cytology/*physiology
Sciatic Nerve/anatomy & histology
Sterol Regulatory Element Binding Protein 1/genetics/*metabolism
Sterol Regulatory Element Binding Protein 2/genetics/metabolism
Tissue Distribution
Pubmed
Web of science
Create date
25/01/2008 16:18
Last modification date
20/08/2019 15:30