The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.

Details

Serval ID
serval:BIB_BBED813F1F73
Type
Article: article from journal or magazin.
Collection
Publications
Title
The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
Journal
Cell
Author(s)
Mermod N., O'Neill E.A., Kelly T.J., Tjian R.
ISSN
0092-8674[print], 0092-8674[linking]
Publication state
Published
Issued date
08/1989
Volume
58
Number
4
Pages
741-753
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Human CTF/NF-I consists of a family of CCAAT box binding proteins that activate both transcription and DNA replication. Analysis of cDNA mutants expressed in E. coli and Drosophila cells reveals that the N-terminal portion of CTF-1 is sufficient for site-specific DNA recognition, protein dimerization, and adenovirus replication. In contrast, transcriptional activation requires an additional C-terminal domain. Furthermore, this transcription domain efficiently activates a heterologous promoter, such as SV40, when fused to the DNA binding domain of Sp1. The CTF C-terminal region consists of an unusual type of transcriptional activation domain containing approximately 25% proline residues. We propose that this proline-rich domain represents a novel class of activators which are distinct from those containing either acidic or glutamine-rich activation motifs. This indicates that transcriptional activation is likely to be mediated by several different mechanisms. In addition, these results suggest that the interactions, and consequently the mechanisms, governing transcriptional activation by CTF are distinct from those mediating DNA replication.
Keywords
Amino Acid Sequence, Animals, Binding Sites, Cloning, Molecular, DNA Mutational Analysis, DNA Replication, DNA-Binding Proteins/genetics, DNA-Binding Proteins/physiology, Drosophila melanogaster/genetics, Macromolecular Substances, Metalloproteins/genetics, Molecular Sequence Data, Proline, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Structure-Activity Relationship, Transcription Factors/physiology, Transcription, Genetic
Pubmed
Web of science
Create date
24/01/2008 10:41
Last modification date
20/08/2019 15:29
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