DNA extraction using the QIAsymphony: Evaluation of PCR inhibitor removal

Details

Serval ID
serval:BIB_BBBA484BA3AF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
DNA extraction using the QIAsymphony: Evaluation of PCR inhibitor removal
Journal
Forensic Science International: Genetics Supplement Series
Author(s)
Castella V., Kummer D., Gehrig C., Hall D.
ISSN
1875-1768
ISSN-L
1875-1768
Publication state
Published
Issued date
2011
Volume
3
Pages
e69-e-70
Language
english
Abstract
The goal of this study was to compare the quantity and purity of DNA extracted from biological tracesusing the QIAsymphony robot with that of the manual QIAamp DNA mini kit currently in use in ourlaboratory. We found that the DNA yield of robot was 1.6-3.5 times lower than that of the manualprotocol. This resulted in a loss of 8% and 29% of the alleles correctly scored when analyzing 1/400 and 1/800 diluted saliva samples, respectively. Specific tests showed that the QIAsymphony was at least 2-16times more efficient at removing PCR inhibitors. The higher purity of the DNA may therefore partlycompensate for the lower DNA yield obtained. No case of cross-contamination was observed amongsamples. After purification with the robot, DNA extracts can be automatically transferred in 96-wellsplates, which is an ideal format for subsequent RT-qPCR quantification and DNA amplification. Lesshands-on time and reduced risk of operational errors represent additional advantages of the robotic platform.
Keywords
Automation, DNA extraction, STR , Real time PCR, Robot,
Create date
28/02/2012 13:32
Last modification date
20/08/2019 15:29
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