A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI
Details
Serval ID
serval:BIB_BB44C3BA1A1F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI
Journal
FEMS Microbiology Letters
ISSN
0378-1097 (Print)
Publication state
Published
Issued date
07/1993
Volume
111
Number
1
Pages
31-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 15
Research Support, Non-U.S. Gov't --- Old month value: Jul 15
Abstract
The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus, was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus.
Keywords
*Allergens
Animals
Aspergillosis/microbiology
Aspergillus fumigatus/*genetics/isolation & purification/pathogenicity
Chromosomes, Fungal
Cloning, Molecular
Cytotoxins/*genetics
DNA, Fungal/genetics/isolation & purification
Fungal Proteins/analysis/biosynthesis/*genetics
Genes, Fungal
Humans
Mice
Plasmids
Restriction Mapping
*Ribonucleases
*Transformation, Genetic
Virulence/genetics
Pubmed
Web of science
Create date
25/01/2008 17:46
Last modification date
20/08/2019 16:29