YscU recognizes translocators as export substrates of the Yersinia injectisome.

Détails

ID Serval
serval:BIB_BB4308686C43
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
YscU recognizes translocators as export substrates of the Yersinia injectisome.
Périodique
The EMBO Journal
Auteur(s)
Sorg I., Wagner S., Amstutz M., Müller S.A., Broz P., Lussi Y., Engel A., Cornelis G.R.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
26
Numéro
12
Pages
3015-3024
Langue
anglais
Résumé
YscU is an essential component of the export apparatus of the Yersinia injectisome. It consists of an N-terminal transmembrane domain and a long cytoplasmic C-terminal domain, which undergoes auto-cleavage at a NPTH site. Substitutions N263A and P264A prevented cleavage of YscU and abolished export of LcrV, YopB and YopD but not of Yop effectors. As a consequence, yscU(N263A) mutant bacteria made needles without the LcrV tip complex and they could not form translocation pores. The graft of the export signal of the effector YopE, at the N-terminus of LcrV, restored LcrV export and assembly of the tip complex. Thus, YscU cleavage is required to acquire the conformation allowing recognition of translocators, which represent an individual category of substrates in the hierarchy of export. In addition, yscU(N263A) mutant bacteria exported reduced amounts of the YscP ruler and made longer needles. Increasing YscP export resulted in needles with normal size, depending on the length of the ruler. Hence, the effect of the yscU(N263A) mutation on needle length was the consequence of a reduced YscP export.

Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Microscopy, Electron, Transmission, Mutation, Protein Binding, Protein Transport, Yersinia/metabolism, Yersinia/ultrastructure
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/10/2017 11:05
Dernière modification de la notice
20/08/2019 16:29
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