Optimization and regeneration kinetics of lymphatic-specific photodynamic therapy in the mouse dermis.

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Serval ID
serval:BIB_BB356E01B697
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Optimization and regeneration kinetics of lymphatic-specific photodynamic therapy in the mouse dermis.
Journal
Angiogenesis
Author(s)
Kilarski W.W., Muchowicz A., Wachowska M., Mężyk-Kopeć R., Golab J., Swartz M.A., Nowak-Sliwinska P.
ISSN
1573-7209 (Electronic)
ISSN-L
0969-6970
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
17
Number
2
Pages
347-357
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish PDF : Original Paper
Abstract
Lymphatic vessels transport fluid, antigens, and immune cells to the lymph nodes to orchestrate adaptive immunity and maintain peripheral tolerance. Lymphangiogenesis has been associated with inflammation, cancer metastasis, autoimmunity, tolerance and transplant rejection, and thus, targeted lymphatic ablation is a potential therapeutic strategy for treating or preventing such events. Here we define conditions that lead to specific and local closure of the lymphatic vasculature using photodynamic therapy (PDT). Lymphatic-specific PDT was performed by irradiation of the photosensitizer verteporfin that effectively accumulates within collecting lymphatic vessels after local intradermal injection. We found that anti-lymphatic PDT induced necrosis of endothelial cells and pericytes, which preceded the functional occlusion of lymphatic collectors. This was specific to lymphatic vessels at low verteporfin dose, while higher doses also affected local blood vessels. In contrast, light dose (fluence) did not affect blood vessel perfusion, but did affect regeneration time of occluded lymphatic vessels. Lymphatic vessels eventually regenerated by recanalization of blocked collectors, with a characteristic hyperplasia of peri-lymphatic smooth muscle cells. The restoration of lymphatic function occurred with minimal remodeling of non-lymphatic tissue. Thus, anti-lymphatic PDT allows control of lymphatic ablation and regeneration by alteration of light fluence and photosensitizer dose.
Pubmed
Web of science
Open Access
Yes
Create date
16/05/2014 16:57
Last modification date
20/08/2019 15:29
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