Article: article from journal or magazin.
Calpain 3 mRNA expression in mice after denervation and during muscle regeneration.
American Journal of Physiology. Cell Physiology
Publication types: Journal Article
Lack of functional calpain 3 in humans is a cause of limb girdle muscular dystrophy, but the function(s) of calpain 3 remain(s) unknown. Special muscle conditions in which calpain 3 is downregulated could yield valuable clues to the understanding of its function(s). We monitored calpain 3 mRNA amounts by quantitative RT-PCR and compared them with those of alpha-skeletal actin mRNA in mouse leg muscles for different types of denervation and muscle injury. Intact muscle denervation reduced calpain 3 mRNA expression by a factor of 5 to 10, while alpha-skeletal actin mRNA was reduced in a slower and less extensive manner. Muscle injury (denervation-devascularization), which leads to muscle degeneration and regeneration, induced a 20-fold decrease in the mRNA level of both calpain 3 and alpha-skeletal actin. Furthermore, whereas in normal muscle and intact denervated muscle, the full-length transcript is the major calpain 3 mRNA, in injured muscle, isoforms lacking exon 6 are predominant during the early regeneration process. These data suggest that muscle condition determines the specific calpain 3 isoform pattern of expression and that calpain 3 expression is downregulated by denervation.
Actins/genetics, Alternative Splicing/physiology, Animals, Apoptosis/physiology, Calpain/genetics, DNA Primers, Gene Expression Regulation, Enzymologic/physiology, Male, Mice, Muscle Denervation, Muscle Fibers, Skeletal/enzymology, Muscle Fibers, Skeletal/pathology, Muscle Proteins, Muscle, Skeletal/pathology, Muscle, Skeletal/physiology, RNA, Messenger/analysis, Regeneration/physiology, Reverse Transcriptase Polymerase Chain Reaction, Sciatic Nerve/physiology, Sciatic Nerve/surgery
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