The purpose of this study is to report allele frequency data of a Huastecos ethnic group population sample (n=89) from La Huasteca (Hidalgo, Mexico) for 12 STR-PCR polymorphisms (HumTH01, HumvWA, D18S51, HumTPOX, D19S433, D16S539, D13S317, D8S1179, D7S820, D5S818, HumFGA and D21S11). No significant deviations from Hardy-Weinberg expectation were found for all short-tandem repeats (STR). From forensic point of view, the heterozygosity value, power of discrimination and the a priori chance exclusion value were calculated.