Specific surface glycan decorations enable antimicrobial peptide resistance in plant-beneficial pseudomonads with insect-pathogenic properties.

Détails

Ressource 1Télécharger: 27727519.pdf (6618.14 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_B99FA6EEAA55
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Specific surface glycan decorations enable antimicrobial peptide resistance in plant-beneficial pseudomonads with insect-pathogenic properties.
Périodique
Environmental microbiology
Auteur(s)
Kupferschmied P., Chai T., Flury P., Blom J., Smits T.H., Maurhofer M., Keel C.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Statut éditorial
Publié
Date de publication
11/2016
Peer-reviewed
Oui
Volume
18
Numéro
11
Pages
4265-4281
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Some plant-beneficial pseudomonads can invade and kill pest insects in addition to their ability to protect plants from phytopathogens. We explored the genetic basis of O-polysaccharide (O-PS, O-antigen) biosynthesis in the representative insecticidal strains Pseudomonas protegens CHA0 and Pseudomonas chlororaphis PCL1391 and investigated its role in insect pathogenicity. Both strains produce two distinct forms of O-PS, but differ in the organization of their O-PS biosynthesis clusters. Biosynthesis of the dominant O-PS in both strains depends on a gene cluster similar to the O-specific antigen (OSA) cluster of Pseudomonas aeruginosa. In CHA0 and other P. protegens strains, the OSA cluster is extensively reduced and new clusters were acquired, resulting in high diversity of O-PS structures, possibly reflecting adaptation to different hosts. CHA0 mutants lacking the short OSA form of O-PS were significantly impaired in insect virulence in Galleria injection and Plutella feeding assays. CHA0, PCL1391, and other insecticidal pseudomonads exhibited high resistance to antimicrobial peptides, including cecropins that are central to insect immune defense. Resistance of both model strains depended on the dominant OSA-type O-PS. Our results suggest that O-antigen is essential for successful insect infection and illustrate, for the first time, its importance in resistance of Pseudomonas to antimicrobial peptides.

Mots-clé
Animals, Antimicrobial Cationic Peptides/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Feeding Behavior, Moths/microbiology, Moths/physiology, O Antigens/biosynthesis, Plant Diseases/parasitology, Plants/microbiology, Plants/parasitology, Pseudomonas/drug effects, Pseudomonas/genetics, Pseudomonas/metabolism, Pseudomonas/pathogenicity, Pseudomonas aeruginosa/drug effects, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Pseudomonas aeruginosa/pathogenicity, Virulence
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/12/2015 14:55
Dernière modification de la notice
09/05/2019 0:20
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