Frequency and cross-reactivity od cytolytic T lymphocyte precursors reacting against male alloantigens

Details

Serval ID
serval:BIB_B9445E898D2D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Frequency and cross-reactivity od cytolytic T lymphocyte precursors reacting against male alloantigens
Journal
Journal of Immunology
Author(s)
Kanagawa  O., Louis  J., Cerottini  J. C.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
05/1982
Volume
128
Number
5
Pages
2362-6
Notes
Journal Article --- Old month value: May
Abstract
It is well established that cytotoxic T lymphocytes (CTL) specific for the male minor histocompatibility antigen (H-Y) are generated by restimulation in vitro of in vivo primed spleen cells from C57BL/6 (H-2b) female mice with syngeneic male spleen cells. When tested on target cells from H-2 different strains, the male-specific C57BL/6 CTL populations exhibited significant lysis of DBA/2 (H-2d), A (H-2a), but not C3H (H-2k), male and female target cells. In an attempt to document this cross-reactivity further at the clonal level, a sensitive technique of limiting dilution analysis was used to determine the specificity of C57BL/6 individual CTL precursors (CTL-P) reactive against the male antigen. The mean frequency of anti-H-Y CTL-P in spleens of primed female mice was about 1/3500. Between one-third to one-tenth of these CTL-P produced a progeny that cross-reacted with H-2d (allogeneic) female target cells. These findings were confirmed by the analysis of the reactivity pattern exhibited by male-specific CTL clones derived by limiting dilution. Of 99 clones tested, 13 were found to cross-react with female DBA/2 target cells. These results thus indicate that a relatively large proportion (greater than 10%) of H-2b CTL-P directed against the H-Y antigen cross-react with target cells expressing H-2d alloantigens in the absence of H-Y antigen.
Keywords
Animals Antibody Formation Cell Differentiation Clone Cells/immunology *Cross Reactions *Cytotoxicity, Immunologic Epitopes Female H-Y Antigen/*immunology Lymphocyte Culture Test, Mixed Male Mice Mice, Inbred A Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred DBA T-Lymphocytes/cytology/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 15:27
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