Stereoselective determination of citalopram and desmethylcitalopram in human plasma and breast milk by liquid chromatography tandem mass spectrometry.

Détails

ID Serval
serval:BIB_B83BFE02B0D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Stereoselective determination of citalopram and desmethylcitalopram in human plasma and breast milk by liquid chromatography tandem mass spectrometry.
Périodique
Journal of pharmaceutical and biomedical analysis
Auteur(s)
Weisskopf E., Panchaud A., Nguyen K.A., Grosjean D., Hascoët J.M., Csajka C., Eap C.B., Ansermot N.
Collaborateur(s)
collaborators of the SSRI-Breast Milk study
ISSN
1873-264X (Electronic)
ISSN-L
0731-7085
Statut éditorial
Publié
Date de publication
30/11/2016
Peer-reviewed
Oui
Volume
131
Pages
233-245
Langue
anglais
Notes
Publication types: Journal Article ; Validation Studies
Publication Status: ppublish
Résumé
A high performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS) method was developed for the simultaneous, stereoselective quantification of the antidepressant citalopram and its active metabolite desmethylcitalopram in human plasma and breast milk. Sample preparation was performed by a two-step approach, including generic protein precipitation with acetonitrile followed by solid phase extraction. Enantiospecific separation of analytes was achieved on a Phenomenex(®) Lux Cellulose-2 column (4.6mm×150mm; 5μm), using reversed phase chromatography conditions characterized by a gradient elution of ammonium acetate buffer (pH 9.0; 20mM) and acetonitrile at a flow rate of 0.6ml/min. The compounds were detected by a tandem quadrupole mass spectrometer equipped with an electrospray ionization source and operating in multiple reaction monitoring mode. The method was fully validated in both biological fluids over a large concentration range of 0.1-100ng/ml for S-(+)- and R-(-)-citalopram, and 0.3-100ng/ml for S-(+)- and R-(-)-desmethylcitalopram. Trueness (90.0-113.3% and 97.1-103.6%), repeatability (0.9-15.9% and 0.9-8.4%) and intermediate precision (1.3-17.8% and 0.9-9.6%) in plasma and breast milk, respectively, meet international guidelines for method validation. Internal standard-normalized matrix effects ranged between 99 and 101% and 98-105%, respectively. The accuracy profiles (total error of trueness and precision) were mostly within the acceptance limits for biological samples defined as ±30%. The method was successfully applied to patient samples in a clinical trial setting.

Mots-clé
Chromatography, High Pressure Liquid/methods, Chromatography, Reverse-Phase/methods, Citalopram/analogs & derivatives, Citalopram/blood, Citalopram/metabolism, Humans, Milk, Human/metabolism, Reproducibility of Results, Solid Phase Extraction, Spectrometry, Mass, Electrospray Ionization/methods, Stereoisomerism, Tandem Mass Spectrometry/methods, Breast milk, Citalopram, Enantiomer, LC–MS/MS, Plasma, Quantification
Pubmed
Web of science
Création de la notice
11/10/2016 15:00
Dernière modification de la notice
20/08/2019 15:26
Données d'usage