Sustained transgene expression using MAR elements.

Détails

ID Serval
serval:BIB_B7D9B1ABC84D
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Sustained transgene expression using MAR elements.
Périodique
Current Gene Therapy
Auteur(s)
Harraghy N., Gaussin A., Mermod N.
ISSN
1566-5232[print], 1566-5232[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
8
Numéro
5
Pages
353-366
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Résumé
Matrix attachment regions (MARs) are DNA sequences that may be involved in anchoring DNA/chromatin to the nuclear matrix and they have been described in both mammalian and plant species. MARs possess a number of features that facilitate the opening and maintenance of euchromatin. When incorporated into viral or non-viral vectors MARs can increase transgene expression and limit position-effects. They have been used extensively to improve transgene expression and recombinant protein production and promising studies on the potential use of MAR elements for mammalian gene therapy have appeared. These illustrate how MARs may be used to mediate sustained or higher levels of expression of therapeutic genes and/or to reduce the viral vector multiplicity of infection required to achieve consistent expression. More recently, the discovery of potent MAR elements and the development of improved vectors for transgene delivery, notably non-viral episomal vectors, has strengthened interest in their use to mediate expression of therapeutic transgenes. This article will describe the progress made in this field, and it will discuss future directions and issues to be addressed.
Mots-clé
Animals, Animals, Genetically Modified, Gene Expression, Gene Silencing, Gene Therapy/methods, Genetic Vectors, Hematopoietic Stem Cells/metabolism, Humans, Matrix Attachment Regions, Models, Genetic, Neurons/metabolism, Plasmids/genetics, T-Lymphocytes/metabolism, Virus Integration, Viruses/genetics
Pubmed
Web of science
Création de la notice
19/03/2009 11:15
Dernière modification de la notice
20/08/2019 15:25
Données d'usage