Keratinocyte growth factor increases transalveolar sodium reabsorption in normal and injured rat lungs.

Details

Serval ID
serval:BIB_B7C498A6B33B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Keratinocyte growth factor increases transalveolar sodium reabsorption in normal and injured rat lungs.
Journal
American journal of respiratory and critical care medicine
Author(s)
Guery B.P., Mason C.M., Dobard E.P., Beaucaire G., Summer W.R., Nelson S.
ISSN
1073-449X (Print)
ISSN-L
1073-449X
Publication state
Published
Issued date
05/1997
Peer-reviewed
Oui
Volume
155
Number
5
Pages
1777-1784
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Keratinocyte growth factor (KGF) prevents alpha-naphthylthiourea (ANTU)-induced permeability edema ex vivo. To explore the mechanisms in this involved effect, we administered KGF (5 mg/kg, intratracheally) 48 h prior to ANTU (50 mg/kg, intraperitoneally). Several groups were studied: phosphate-buffered saline/dimethylsulfoxide (PBS/DMSO) (vehicles), PBS/ANTU, and KGF/ANTU. At 90 min after ANTU injection the lungs were removed, ventilated, and perfused ex vivo for 180 min. Quantification of fluorescein isothiocyanate (FITC)-labeled dextran in bronchoalveolar lavage fluid (BALF) was used to assess alveolar capillary barrier permeability. KGF attenuated ANTU-induced edema and blockade of sodium transport, with ouabain (10(-3) M) or amiloride (10(-4) M) added ex vivo reversed this effect. FITC-dextran was increased in the PBS/ANTU group as compared with the PBS/DMSO group, indicating permeability edema. In the KGF/ANTU group, there was concentration of BALF FITC-dextran, consistent with permeability edema and increased alveolar fluid export. Albumin space measurements showed similar increases in permeability in the PBS/ANTU and KGF/ANTU groups. Extravascular lung water (measured with radiolabeled erythrocytes) was decreased in the KGF/ANTU group. Following KGF pretreatment, uninjured lungs exported more intratracheal PBS than normal lungs following terbutaline stimulation ex vivo. In conclusion, KGF, through type II alveolar pneumocyte hyperplasia with increased sodium-potassium-adenosine triphosphatase (Na,K-ATPase) activity, attenuated ANTU-induced edema formation by potentiating alveolar fluid clearance.
Keywords
Absorption, Amiloride/pharmacology, Animals, Biological Transport, Bronchoalveolar Lavage Fluid/chemistry, Capillary Permeability, Dimethyl Sulfoxide/pharmacology, Enzyme Activation, Extravascular Lung Water/metabolism, Fibroblast Growth Factor 10, Fibroblast Growth Factor 7, Fibroblast Growth Factors, Growth Substances/pharmacology, Ouabain/pharmacology, Pulmonary Alveoli/metabolism, Pulmonary Alveoli/pathology, Pulmonary Edema/chemically induced, Pulmonary Edema/metabolism, Pulmonary Edema/pathology, Pulmonary Edema/physiopathology, Rats, Sodium/metabolism, Sodium Chloride, Sodium-Potassium-Exchanging ATPase/metabolism, Thiourea/analogs & derivatives
Pubmed
Web of science
Create date
29/04/2021 10:59
Last modification date
17/07/2023 14:31
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