Indirect coupling of urate and p-aminohippurate transport to sodium in human brush-border membrane vesicles

Détails

ID Serval
serval:BIB_B6D9F23DF0C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Indirect coupling of urate and p-aminohippurate transport to sodium in human brush-border membrane vesicles
Périodique
American Journal of Physiology
Auteur(s)
Roch-Ramel  F., Guisan  B., Schild  L.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
01/1996
Volume
270
Numéro
1 Pt 2
Pages
F61-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
[14C]urate and p-[14C]aminohippurate (PAH) uptake by human brush-border membrane vesicles (BBMV) were measured in the presence of an inwardly oriented sodium gradient. No direct sodium cotransport was observed. Indirect [14C]urate coupling to sodium transport was demonstrated by cis-stimulation of [14C]urate with nicotinate or pyrazinoate (PZA) in the extravesicular medium but not by adding lactate, alpha-ketoglutarate, or beta-hydroxybutyrate. Indirect sodium coupling of [14C]PAH uptake was observed only when alpha-ketoglutarate was added to the extravesicular medium, a mechanism similar to that of basolateral membranes. The ability for PZA (and nicotinate) to cis-stimulate urate uptake was correlated with a high apparent affinity for the urate/anion exchanger. In urate-loaded vesicles, for identical medium concentrations, [14C]PZA uptake via the urateanion exchanger was 10 times higher than [14C]lactate uptake. Such high PZA affinity for the urate exchanger, working in parallel with PZA sodium cotransport can account for the stimulation of urate reabsorption by PZA in vivo.
Mots-clé
Aged Biological Transport Humans Kidney/*metabolism Microvilli/metabolism Middle Aged Pyrazinamide/analogs & derivatives/metabolism Sodium/*metabolism Sodium Lactate/metabolism Uric Acid/*metabolism p-Aminohippuric Acid/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 13:56
Dernière modification de la notice
20/08/2019 16:25
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