Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy.

Détails

ID Serval
serval:BIB_B6AFEA5BE1AB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy.
Périodique
Journal of Biophotonics
Auteur(s)
Pavillon N., Benke A., Boss D., Moratal C., Kühn J., Jourdain P., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
1864-0648[electronic], 1864-063X[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
3
Numéro
7
Pages
432-436
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death.
Pubmed
Web of science
Création de la notice
05/07/2010 14:48
Dernière modification de la notice
20/08/2019 15:25
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