A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco

Details

Serval ID
serval:BIB_B611D3F6286B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco
Journal
Plant Cell
Author(s)
Hauffe  K. D., Paszkowski  U., Schulze-Lefert  P., Hahlbrock  K., Dangl  J. L., Douglas  C. J.
ISSN
1040-4651
Publication state
Published
Issued date
05/1991
Peer-reviewed
Oui
Volume
3
Number
5
Pages
435-43
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Abstract
The 4CL-1 gene is one of two highly homologous parsley genes encoding 4-coumarate:coenzyme A ligase, a key enzyme of general phenylpropanoid metabolism. Expression of these genes is essential for the biosynthesis of both defense-related and developmentally required phenylpropanoid derivatives. We examined the developmental regulation of the 4CL-1 promoter by analyzing the expression of 4CL-1-beta-glucuronidase fusions in transgenic tobacco plants. A 597-base pair 4CL-1 promoter fragment specified histochemically detectable expression in a complex array of vegetative and floral tissues and cell types. The activity of a series of 5' deleted promoter fragments was analyzed in parsley protoplasts and transgenic tobacco plants. Deletions past -210 base pairs led to a drastic decline in beta-glucuronidase activity in protoplasts and loss of tissue-specific expression in transgenic tobacco. These results were put into the context of potential protein-DNA interactions by in vivo footprint analysis of the 4CL-1 promoter in parsley cells. Loss of promoter activity in parsley protoplasts and transgenic tobacco was correlated with the deletion or disruption of the distal portion of a large (100-base pair) footprinted region within the first 200 base pairs of the 4CL-1 promoter.
Keywords
Base Composition Base Sequence Coenzyme A Ligases/*genetics/metabolism Dna *Gene Expression Regulation Genes, Plant Molecular Sequence Data Organ Specificity/genetics Plants/enzymology/*genetics Plants, Genetically Modified Plants, Toxic *Promoter Regions (Genetics) Protoplasts Restriction Mapping Tobacco
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 19:47
Last modification date
20/08/2019 15:24
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