Limiting dilution analysis of alloantigen-reactive T lymphocytes. VI. Ontogeny of cytolytic T lymphocyte precursors in the thymus
Details
Serval ID
serval:BIB_B5F454BA6595
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Limiting dilution analysis of alloantigen-reactive T lymphocytes. VI. Ontogeny of cytolytic T lymphocyte precursors in the thymus
Journal
Thymus
ISSN
0165-6090 (Print)
Publication state
Published
Issued date
02/1981
Volume
2
Number
4-5
Pages
245-55
Notes
Journal Article --- Old month value: Feb
Abstract
The ontogeny of development of precursors of cytolytic T lymphocytes (CTL-P) in the murine thymus has been investigated. Fetal or newborn thymocytes harvested from C57BL/6 mice were cultured with irradiated allogeneic (DBA/2) spleen cells in limiting dilution mixed leukocyte microcultures and assayed for cytotoxicity against 51Cr-labeled P-815 (DBA/2) tumor target cells. By applying Poisson statistics to these data, minimal estimates of thymic CTL-P frequencies were determined. Whereas CTL-P were barely detectable in fetal or newborn thymuses (3-19 CTL-P/10(6) cells), their frequency increased dramatically (to 44-127 10(6) cells) within 24 h of birth. In comparison, control adult (day 70) thymuses contained 300-400 CTL-P/10(6) cells. Mixing experiments indicated that the low CTL-P frequencies observed in newborn thymuses could not be attributed to a suppressive phenomenon. These data provide strong indirect evidence for functional maturation of CTL-P within the neonatal thymus.
Keywords
Animals
Animals, Newborn
*Cytotoxicity, Immunologic
Female
Indicator Dilution Techniques
*Isoantigens
Lymphocyte Culture Test, Mixed
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Species Specificity
T-Lymphocytes/*immunology
T-Lymphocytes, Regulatory/immunology
Thymus Gland/*immunology
Pubmed
Web of science
Create date
28/01/2008 12:14
Last modification date
20/08/2019 16:24