Limiting dilution analysis of alloantigen-reactive T lymphocytes. VI. Ontogeny of cytolytic T lymphocyte precursors in the thymus

Details

Serval ID
serval:BIB_B5F454BA6595
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Limiting dilution analysis of alloantigen-reactive T lymphocytes. VI. Ontogeny of cytolytic T lymphocyte precursors in the thymus
Journal
Thymus
Author(s)
Widmer  M. B., MacDonald  H. R., Cerottini  J. C.
ISSN
0165-6090 (Print)
Publication state
Published
Issued date
02/1981
Volume
2
Number
4-5
Pages
245-55
Notes
Journal Article --- Old month value: Feb
Abstract
The ontogeny of development of precursors of cytolytic T lymphocytes (CTL-P) in the murine thymus has been investigated. Fetal or newborn thymocytes harvested from C57BL/6 mice were cultured with irradiated allogeneic (DBA/2) spleen cells in limiting dilution mixed leukocyte microcultures and assayed for cytotoxicity against 51Cr-labeled P-815 (DBA/2) tumor target cells. By applying Poisson statistics to these data, minimal estimates of thymic CTL-P frequencies were determined. Whereas CTL-P were barely detectable in fetal or newborn thymuses (3-19 CTL-P/10(6) cells), their frequency increased dramatically (to 44-127 10(6) cells) within 24 h of birth. In comparison, control adult (day 70) thymuses contained 300-400 CTL-P/10(6) cells. Mixing experiments indicated that the low CTL-P frequencies observed in newborn thymuses could not be attributed to a suppressive phenomenon. These data provide strong indirect evidence for functional maturation of CTL-P within the neonatal thymus.
Keywords
Animals Animals, Newborn *Cytotoxicity, Immunologic Female Indicator Dilution Techniques *Isoantigens Lymphocyte Culture Test, Mixed Male Mice Mice, Inbred C57BL Mice, Inbred DBA Species Specificity T-Lymphocytes/*immunology T-Lymphocytes, Regulatory/immunology Thymus Gland/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:14
Last modification date
20/08/2019 15:24
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