Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.

Details

Serval ID
serval:BIB_B5ACBD42DCAE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.
Journal
Journal of Bacteriology
Author(s)
van der Meer J.R., Frijters A.C., Leveau J.H., Eggen R.I., Zehnder A.J., de Vos W.M.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Publication state
Published
Issued date
1991
Peer-reviewed
Oui
Volume
173
Number
12
Pages
3700-3708
Language
english
Abstract
Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing and expression studies with Escherichia coli and pET8c and appeared to encode a 32-kDa protein. The activity of the tcbR gene product was analyzed in Pseudomonas putida KT2442, in which it appeared to function as a positive regulator of tcbC expression. Protein extracts of both E. coli overproducing TcbR and Pseudomonas sp. strain P51 showed specific DNA binding to the 150-bp region that is located between the tcbR and tcbC genes. Primer extension mapping demonstrated that the transcription start sites of tcbR and tcbC are located in this region and that the divergent promoter sequences of both genes overlap. Amino acid sequence comparisons indicated that TcbR is a member of the LysR family of transcriptional activator proteins and shares a high degree of homology with other activator proteins involved in regulating the metabolism of aromatic compounds.
Keywords
Amino Acid Sequence, Base Sequence, Catechol 1,2-Dioxygenase, Catechols/metabolism, DNA, Bacterial/genetics, Dioxygenases, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Gene Expression Regulation, Bacterial, Genes, Bacterial, Molecular Sequence Data, Operon, Oxidation-Reduction, Oxygenases/metabolism, Plasmids, Promoter Regions, Genetic, Pseudomonas/genetics, Regulatory Sequences, Nucleic Acid, Sequence Alignment, Trans-Activators, Transcription, Genetic
Pubmed
Web of science
Create date
21/01/2008 13:35
Last modification date
20/08/2019 15:24
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