Induction of CTL in vivo by major histocompatibility complex class I-peptide complexes covalently associated on the cell surface.

Details

Serval ID
serval:BIB_B566852E9FF3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Induction of CTL in vivo by major histocompatibility complex class I-peptide complexes covalently associated on the cell surface.
Journal
European journal of immunology
Author(s)
Anjuère F., Horvath C., Cerottini J.C., Luescher I.F.
ISSN
0014-2980
Publication state
Published
Issued date
1995
Peer-reviewed
Oui
Volume
25
Number
6
Pages
1535-1540
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The identification of endogenously produced antigenic peptides presented by MHC class I molecules has opened the way to peptide-based strategies for CTL induction in vivo. Here we demonstrate that the induction in vivo of CTL directed against naturally processed antigens can be triggered by injection of syngeneic cells expressing covalent major histocompatibility complex class I-peptide complexes. In the model system used, the induction of HLA-Cw3 specific cytotoxic T lymphocytes (CTL) in mice by cell surface-associated, covalent H-2Kd (Kd)-Cw3 peptide complexes was investigated. The Kd-restricted Cw3 peptide 170-179 (RYLKNGKETL), which mimics the major natural epitope recognized by Cw3-specific CTL in H-2d mice, was converted to a photoreactive derivative by replacing Arg-170 with N-beta-(4-azidosalicyloyl)-L-2,3-diaminopropionic acid. This peptide derivative was equivalent to the parental Cw3 peptide in terms of binding to Kd molecules and recognition by Cw3-specific CTL clones and could be cross-linked efficiently and selectively to Kd molecules on the surface of Con A-stimulated spleen cells from H-2d mice. Photocross-linking prevented the rapid dissociation of Kd-peptide derivative complexes that takes place under physiological conditions. Cultures of spleen cells or peritoneal exudate cells from mice inoculated i.p. with peptide-pulsed and photocross-linked cells developed a strong CTL response following antigenic stimulation in vitro. The cultured cells efficiently lysed not only target cells sensitized with the Cw3 170-179 peptide but also target cells transfected with the Cw3 gene. Moreover, their TCR preferentially expressed V beta 10 and J alpha pHDS58 segments as well as conserved junctional sequences, as has been observed previously in Cw3-specific CTL responses. In contrast, no Cw3-specific CTL response could be obtained in cultures derived from mice injected with Con A-stimulated spleen cells pulsed with the peptide derivative without photocross-linking.
Keywords
Amino Acid Sequence, Animals, Antigens, Surface/immunology, Cells, Cultured, Histocompatibility Antigens Class I/immunology, Histocompatibility Antigens Class I/metabolism, Immunization, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Peptides/chemistry, Peptides/immunology, Spleen/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Create date
28/01/2008 11:20
Last modification date
20/08/2019 15:23
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