CK2 phosphorylation of Bdp1 executes cell cycle-specific RNA polymerase III transcription repression.

Détails

ID Serval
serval:BIB_B555224FBCDB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CK2 phosphorylation of Bdp1 executes cell cycle-specific RNA polymerase III transcription repression.
Périodique
Molecular Cell
Auteur(s)
Hu P., Samudre K., Wu S., Sun Y., Hernandez N.
ISSN
1097-2765[print], 1097-2765[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
16
Numéro
1
Pages
81-92
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
RNA polymerase III (pol III) transcription from the human U6 snRNA promoter can be reconstituted with the recombinant factors SNAPc and Brf2-TFIIIB combined with purified pol III. In this system, CK2 treatment of the pol III complex is required for transcription, whereas treatment of Brf2-TFIIIB is inhibitory. Here we show that CK2 inhibits Brf2-TFIIIB by specifically phosphorylating its Bdp1 component. Bdp1 is phosphorylated by CK2 during mitosis, and this is accompanied by Bdp1 dissociation from the U6 promoter and from chromatin in general and by transcription repression. Remarkably, whereas inhibition of CK2 in mitotic extracts restores pol III transcription, inhibition of CK2 in active S phase extracts debilitates transcription. Thus, CK2 is directed to phosphorylate different targets within the basal pol III transcription machinery at different times during the cell cycle, with opposite transcriptional effects.
Mots-clé
Casein Kinase II, Cell Cycle/physiology, Chromatin/physiology, Conserved Sequence, Gene Expression Regulation/physiology, Humans, Mitosis/physiology, Mutation, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Phosphorylation, Promoter Regions, Genetic, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases/metabolism, RNA Polymerase III/metabolism, Transcription Factor TFIIIB, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 16:23
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