Avoiding epigenetic variations in transgene expression with genetic insulators

Détails

ID Serval
serval:BIB_B4B957503854
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Poster: résume de manière illustrée et sur une page unique les résultats d'un projet de recherche. Les résumés de poster doivent être entrés sous "Abstract" et non "Poster".
Collection
Publications
Titre
Avoiding epigenetic variations in transgene expression with genetic insulators
Titre de la conférence
ESGCT, DGGT, GSZ, and ISCT 2009 Poster Presentations
Auteur(s)
Gaussin A., Duros C., Artus A., Auclair C., Cohen-Haguenauer O., Mermod N.
Organisation
Abstracts, Combined Meeting 2009, November 20-25, 2009, Hannover, Germany, Convention Center at Hannover Fairground XVIIth Annual Congress of the European Society of Gene and Cell Therapy, 16th Annual Meeting of the German Society of Gene Therapy, 4th Annual Congress of the German Society for Stem Cell Research, Co-organized by the European Branch of the International Society for Cellular Therapy
ISBN
1043-0342
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
20
Série
Human Gene Therapy
Pages
1506-1506
Langue
anglais
Notes
Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research Hannover, GERMANY, NOV 21-25, 2009 P244
Résumé
Gene transfer that relies on integrating vectors often suffers
from epigenetic or regulatory effects that influence the expression
of the therapeutic gene and=or of cellular genes located
near the vector integration site in the chromosome.
Insulator elements act to block gene activation by enhancers,
while chromatin domain boundary or barrier sequences
prevent gene-silencing effects. At present, the modes of action
of insulator and barriers are poorly understood, and their use
in the context of gene therapies remains to be documented.
Using combinations of reporter genes coding for indicator
fluorescent proteins, we constructed assay systems that allow
the quantification of the insulator or of the barrier activities of
genetic elements in individual cells. This presentation will
illustrate how these assay systems were used to identify short
DNA elements that can insulate nearby genes from activation
by viral vector enhancer elements, and=or that can block the
propagation of a silent chromatin structure that leads to gene
silencing. We will show that small elements of the order of
100-400 nucleotides can be designed to achieve both insulator
and boundary function, as needed for safer integrating viral
vectors.
Web of science
Création de la notice
15/06/2010 10:47
Dernière modification de la notice
20/08/2019 15:23
Données d'usage