Soft X-irradiation influences the integrity of Langerhans cells. A histochemical and immunohistological study

Détails

ID Serval
serval:BIB_B4A6CCE2FBC3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Soft X-irradiation influences the integrity of Langerhans cells. A histochemical and immunohistological study
Périodique
Dermatologica
Auteur(s)
Groh  V., Meyer  J. C., Panizzon  R., Zortea-Caflisch  C.
ISSN
0011-9075 (Print)
Statut éditorial
Publié
Date de publication
1984
Volume
168
Numéro
2
Pages
53-60
Notes
Journal Article
Résumé
This report defines the influence of field soft X-ray irradiation on the integrity of epidermal Langerhans cells (LC) in the guinea pig and mouse systems. Male albino (Rockefeller strain) and piebald F 1 (2 X 13 strain) guinea pigs as well as C3H mice (H-2Kk) were exposed to one single shot of different dosages of soft X-rays [80, 1,200, 1,600, and 3,200 R; 30 kV, 0.5 mm aluminium filter, FSD (focus-skin distance) 12 cm]. 1 and 4 weeks after exposure, skin specimens were taken from the irradiated skin. The demonstration and evaluation of LC was performed basing on their expression of specific histochemical (ATPase) and functional immunologic markers (Ia antigens). Soft X-irradiation had pronounced effects on number and structure of ATPase- and Ia-antigen-positive cells. In the mouse system, 1 week after exposure to 800 and 1,200 R ATPase-positive cells and, in a more or less parellel manner, Ia-antigen-positive cells () were reduced to 74% (74%) and 70% (68%), respectively, and 4 weeks after exposure to 49% (47%) and 43% (40%), respectively. In the guinea pig system one single shot of 1,600 or 3,200 R produced, respectively, a 35% or a 38% reduction of ATPase-positive cells. Prolonged survival did not result in a further depletion of ATPase-positive cells.
Mots-clé
Adenosine Triphosphatases/metabolism Animals Cell Count Cell Survival Fluorescent Antibody Technique Guinea Pigs Histocompatibility Antigens Class II/analysis Langerhans Cells/enzymology/immunology/*radiation effects Male Mice Mice, Inbred C3H Skin/cytology/*radiation effects X-Rays
Pubmed
Web of science
Création de la notice
25/01/2008 16:54
Dernière modification de la notice
20/08/2019 15:23
Données d'usage