Vaccination against Staphylococcus aureus experimental endocarditis using recombinant Lactococcus lactis expressing ClfA or FnbpA.

Détails

ID Serval
serval:BIB_B47DDAD0304E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Vaccination against Staphylococcus aureus experimental endocarditis using recombinant Lactococcus lactis expressing ClfA or FnbpA.
Périodique
Vaccine
Auteur(s)
Veloso T.R., Mancini S., Giddey M., Vouillamoz J., Que Y.A., Moreillon P., Entenza J.M.
ISSN
1873-2518 (Electronic)
ISSN-L
0264-410X
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
33
Numéro
30
Pages
3512-3517
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Staphylococcus aureus is a major cause of serious infections in humans and animals and a vaccine is becoming a necessity. Lactococcus lactis is a non-pathogenic bacterium that can be used as a vector for the delivery of antigens. We investigated the ability of non-living L. lactis heterologously expressing S. aureus clumping factor A (ClfA) and fibronectin-binding protein A (FnbpA), alone or together, to elicit an immune response in rats and protect them from S. aureus experimental infective endocarditis (IE). L. lactis ClfA was used for immunization against S. aureus Newman (expressing ClfA but not FnbpA), while L. lactis ClfA, L. lactis FnbpA, as well as L. lactis ClfA/FnbpA, were used against S. aureus P8 (expressing ClfA and FnbpA). Vaccination of rats with L. lactis ClfA elicited antibodies that inhibited binding of S. aureus Newman to fibrinogen, triggered the production of IL-17A and conferred protection to 13/19 (68%) of the animals from IE (P<0.05). Immunization with L. lactis ClfA, L. lactis FnbpA or L. lactis ClfA/FnbpA also produced antibodies against the target proteins, but these did not prevent binding of S. aureus P8 to fibrinogen or fibronectin and did not protect animals against S. aureus P8 IE. Moreover, immunization with constructs containing FnbpA did not increase IL-17A production. These results indicate that L. lactis is a valuable antigen delivery system able to elicit efficient humoral and cellular responses. However, the most appropriate antigens affording protection against S. aureus IE are yet to be elucidated.
Mots-clé
Adhesins, Bacterial/genetics, Adhesins, Bacterial/immunology, Animals, Antibodies, Bacterial/blood, Coagulase/genetics, Coagulase/immunology, Disease Models, Animal, Drug Carriers, Endocarditis/immunology, Endocarditis/prevention & control, Female, Fibronectins/metabolism, Lactococcus lactis/genetics, Rats, Wistar, Recombinant Proteins/genetics, Recombinant Proteins/immunology, Staphylococcal Vaccines/administration & dosage, Staphylococcal Vaccines/genetics, Staphylococcus aureus/genetics, Staphylococcus aureus/immunology, Treatment Outcome, Vaccination/methods
Pubmed
Web of science
Création de la notice
08/08/2015 16:11
Dernière modification de la notice
03/03/2018 20:42
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