Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease

Détails

ID Serval
serval:BIB_B4764088ED61
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease
Périodique
Gastroenterology
Auteur(s)
Michetti  P., Corthesy-Theulaz  I., Davin  C., Haas  R., Vaney  A. C., Heitz  M., Bille  J., Kraehenbuhl  J. P., Saraga  E., Blum  A. L.
ISSN
0016-5085 (Print)
Statut éditorial
Publié
Date de publication
10/1994
Volume
107
Numéro
4
Pages
1002-11
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
BACKGROUND/AIMS: Because Helicobacter pylori is a potentially dangerous human pathogen, the protective potential of oral immunization with H. pylori urease and its subunits was evaluated in an animal model. METHODS: Mice were orally immunized with H. pylori sonicate, urease, or recombinant enzymatically inactive urease subunits and then challenged with Helicobacter felis. Control mice were sham-immunized. RESULTS: H. felis colonization was present 5 days after challenge in 9 of 10 sham-immunized, 6 of 9 sonicate-immunized, and 3 of 10 urease-immunized animals (P = 0.031 vs. sham-immunized). Twelve days after challenge, urease B-immunized mice had a weaker colonization than sham-immunized controls, whereas urease A had no effect. After 70 days, most urease A- and urease B-immunized mice had cleared the colonization (10/17: P = 0.0019; 16/20: P = 0.00002 vs. sham-immunized). In urease B-immunized animals, protection was often associated with corpus gastritis. CONCLUSIONS: Oral immunization with H. pylori urease protects mice against H. felis infection. Enzymatically inactive urease A and B subunits contain protective epitopes. It is unclear whether protection depends on the development of a mononuclear inflammatory response in the gastric corpus. Our observations should encourage the development of a human vaccine.
Mots-clé
Administration, Oral Animals Antibodies, Monoclonal/immunology Cross Reactions Female Gastritis/pathology Helicobacter Infections/*prevention & control Helicobacter pylori/*enzymology *Immunization Mice Mice, Inbred BALB C Recombinant Proteins Stomach/pathology Urease/administration & dosage/classification/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 17:02
Dernière modification de la notice
03/03/2018 20:42
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