Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_B463362881C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity.
Périodique
Breeding Science
Auteur(s)
Li C., Liu C., Ma X., Wang A., Duan R., Nawrath C., Komatsuda T., Chen G.
ISSN
1344-7610 (Print)
ISSN-L
1344-7610
Statut éditorial
Publié
Date de publication
2015
Volume
65
Numéro
4
Pages
327-332
Langue
anglais
Résumé
The cuticle covers the aerial parts of land plants, where it serves many important functions, including water retention. Here, a recessive cuticle mutant, eceriferum-ym (cer-ym), of Hordeum vulgare L. (barley) showed abnormally glossy spikes, sheaths, and leaves. The cer-ym mutant plant detached from its root system was hypersensitive to desiccation treatment compared with wild type plants, and detached leaves of mutant lost 41.8% of their initial weight after 1 h of dehydration under laboratory conditions, while that of the wild type plants lost only 7.1%. Stomata function was not affected by the mutation, but the mutant leaves showed increased cuticular permeability to water, suggesting a defective leaf cuticle, which was confirmed by toluidine blue staining. The mutant leaves showed a substantial reduction in the amounts of the major cutin monomers and a slight increase in the main wax component, suggesting that the enhanced cuticle permeability was a consequence of cutin deficiency. cer-ym was mapped within a 0.8 cM interval between EST marker AK370363 and AK251484, a pericentromeric region on chromosome 4H. The results indicate that the desiccation sensitivity of cer-ym is caused by a defect in leaf cutin, and that cer-ym is located in a chromosome 4H pericentromeric region.
Mots-clé
eceriferum-mutant, cuticle, cutin, desiccation resistance, genetic mapping, wax
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/11/2015 9:06
Dernière modification de la notice
09/05/2019 0:03
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