Measurement of biologically available naphthalene in gas and aqueous phases by use of a Pseudomonas putida biosensor.

Détails

ID Serval
serval:BIB_B45D4D4D073E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Measurement of biologically available naphthalene in gas and aqueous phases by use of a Pseudomonas putida biosensor.
Périodique
Applied and Environmental Microbiology
Auteur(s)
Werlen C., Jaspers M.C., van der Meer J.R.
ISSN
0099-2240[print], 0099-2240[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
70
Numéro
1
Pages
43-51
Langue
anglais
Résumé
Genetically constructed microbial biosensors for measuring organic pollutants are mostly applied in aqueous samples. Unfortunately, the detection limit of most biosensors is insufficient to detect pollutants at low but environmentally relevant concentrations. However, organic pollutants with low levels of water solubility often have significant gas-water partitioning coefficients, which in principle makes it possible to measure such compounds in the gas rather than the aqueous phase. Here we describe the first use of a microbial biosensor for measuring organic pollutants directly in the gas phase. For this purpose, we reconstructed a bioluminescent Pseudomonas putida naphthalene biosensor strain to carry the NAH7 plasmid and a chromosomally inserted gene fusion between the sal promoter and the luxAB genes. Specific calibration studies were performed with suspended and filter-immobilized biosensor cells, in aqueous solution and in the gas phase. Gas phase measurements with filter-immobilized biosensor cells in closed flasks, with a naphthalene-contaminated aqueous phase, showed that the biosensor cells can measure naphthalene effectively. The biosensor cells on the filter responded with increasing light output proportional to the naphthalene concentration added to the water phase, even though only a small proportion of the naphthalene was present in the gas phase. In fact, the biosensor cells could concentrate a larger proportion of naphthalene through the gas phase than in the aqueous suspension, probably due to faster transport of naphthalene to the cells in the gas phase. This led to a 10-fold lower detectable aqueous naphthalene concentration (50 nM instead of 0.5 micro M). Thus, the use of bacterial biosensors for measuring organic pollutants in the gas phase is a valid method for increasing the sensitivity of these valuable biological devices.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Biosensing Techniques, Chemiluminescent Measurements, Culture Media, Environmental Monitoring/methods, Gases, Luciferases/genetics, Luciferases/metabolism, Naphthalenes/chemistry, Naphthalenes/metabolism, Pseudomonas putida/genetics, Pseudomonas putida/growth & development, Transcription Factors/genetics, Transcription Factors/metabolism, Water
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 14:36
Dernière modification de la notice
09/05/2019 0:03
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