Optical probing of sodium dynamics in neurons and astrocytes.

Détails

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_B42E89C7D790
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Optical probing of sodium dynamics in neurons and astrocytes.
Périodique
Neuroimage
Auteur(s)
Lamy C.M., Chatton J.Y.
ISSN
1095-9572 (Electronic)
ISSN-L
1053-8119
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
58
Numéro
2
Pages
572-578
Langue
anglais
Résumé
Changes in intracellular Na(+) concentration underlie essential neurobiological processes, but few reliable tools exist for their measurement. Here we characterize a new synthetic Na(+)-sensitive fluorescent dye, Asante Natrium Green (ANG), with unique properties. This indicator was excitable in the visible spectrum and by two-photon illumination, suffered little photobleaching and located to the cytosol were it remained for long durations without noticeable unwanted effects on basic cell properties. When used in brain tissue, ANG yielded a bright fluorescent signal during physiological Na(+) responses both in neurons and astrocytes. Synchronous electrophysiological and fluorometric recordings showed that ANG produced accurate Na(+) measurement in situ. This new Na(+) indicator opens innovative ways of probing neuronal circuits.
Mots-clé
Action Potentials/physiology, Algorithms, Animals, Astrocytes/metabolism, Cells, Cultured, Cerebral Cortex/cytology, Computer Simulation, Electrophysiological Phenomena, Fluorescent Dyes, Kinetics, Mice, Mice, Inbred C57BL, Microscopy, Confocal, Microscopy, Fluorescence, Neurons/metabolism, Patch-Clamp Techniques, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Sodium/metabolism, Somatosensory Cortex/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/08/2011 8:06
Dernière modification de la notice
09/05/2019 0:02
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