Control of the proliferation of activated CD4+ T cells by connexins.

Détails

ID Serval
serval:BIB_B3CEEACDBB63
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Control of the proliferation of activated CD4+ T cells by connexins.
Périodique
Journal of Leukocyte Biology
Auteur(s)
Oviedo-Orta E., Perreau M., Evans W.H., Potolicchio I.
ISSN
1938-3673[electronic], 0741-5400[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
88
Numéro
1
Pages
79-86
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
As expression of Cxs in cells of the immune system increases upon cellular activation, we investigated whether Cxs and especially CxHcs play a major role during T cell-mediated responses. In particular, we studied the expression of Cx43Hc following CD4(+) T cell stimulation using flow cytometry, real-time PCR, and Western blot analysis. We showed that expression of Cx43 and its phosphorylated isoforms increased in response to the engagement of CD3 and CD28. Cx43Hcs were found to be involved in sustaining proliferation of T cells, as assessed by cell cycle staining, thymidine incorporation assays, and CFSE analysis of cells exposed to mimetic peptide inhibitors of the plasma membrane Cx channels and antibodies generated to an extracellular region of Cx. The reduction of T cell proliferation mediated by Cx channel inhibitors suppressed cysteine uptake but not cytokine production. We conclude that upon antigen recognition, T cells require CxHc to sustain their clonal expansion.
Mots-clé
CD4-Positive T-Lymphocytes/cytology, CD4-Positive T-Lymphocytes/immunology, Cell Proliferation, Cells, Cultured, Connexin 43/physiology, Connexins/drug effects, Connexins/physiology, Gap Junctions/physiology, Humans, Lymphocyte Activation
Pubmed
Web of science
Création de la notice
19/07/2010 15:41
Dernière modification de la notice
03/03/2018 20:40
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