Autoantibodies to the extracellular and intracellular domain of bullous pemphigoid 180, the putative key autoantigen in bullous pemphigoid, belong predominantly to the IgG1 and IgG4 subclasses

Détails

ID Serval
serval:BIB_B3B204DDE8C1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Autoantibodies to the extracellular and intracellular domain of bullous pemphigoid 180, the putative key autoantigen in bullous pemphigoid, belong predominantly to the IgG1 and IgG4 subclasses
Périodique
British Journal of Dermatology
Auteur(s)
Laffitte  E., Skaria  M., Jaunin  F., Tamm  K., Saurat  J. H., Favre  B., Borradori  L.
ISSN
0007-0963 (Print)
Statut éditorial
Publié
Date de publication
04/2001
Volume
144
Numéro
4
Pages
760-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
BACKGROUND: Autoantibodies to the extracellular domain (ECD) of bullous pemphigoid (BP) antigen 180 (BP180) are thought to play a crucial part in the pathophysiology of BP. OBJECTIVES: As the various IgG subclasses have different biological properties, we have sought to assess the relative isotype distribution of IgG to BP180 and their reactivity against the ECD and intracellular domain (ICD) of BP180. METHODS: The reactivity of 27 sera from patients with BP was assayed by immunoblotting against recombinant proteins covering the ECD and ICD of BP180. RESULTS: Twenty-seven (100%) and 21 (77%) of 27 BP sera, respectively, contained IgG1 and IgG4 autoantibodies binding to the ECD of BP180. Fourteen (82%) and six (35%) of the 17 BP sera that were reactive with the ICD of BP180 had autoantibodies of the IgG1 and IgG4 subclass, respectively. The profile of the isotype restriction appeared to be similar when the response to the ECD vs. that to the ICD was compared. IgG2 and IgG3 reactivity with BP180 was found less frequently. Patients with BP of longer duration showed a tendency to have, in addition to IgG1, an IgG4 response. CONCLUSIONS: Consistent with prior evidence indicating that subepidermal blister formation in BP is dependent upon complement activation, the frequent finding of complement-fixing IgG1 autoantibodies to both the ECD and ICD of BP180 might have pathogenic relevance in BP. These findings provide new insights relevant for our understanding of the immune response to BP180, the putative key autoantigen in BP.
Mots-clé
Aged Aged, 80 and over Animals Antigen-Antibody Reactions/immunology Autoantibodies/*blood Autoantigens/*immunology COS Cells Cercopithecus aethiops Humans Immunoglobulin G/*blood Infant Middle Aged Pemphigoid, Bullous/*immunology/pathology Recombinant Proteins/immunology Time Factors
Pubmed
Web of science
Création de la notice
25/01/2008 17:42
Dernière modification de la notice
03/03/2018 20:40
Données d'usage