Release of N-acetylaspartylglutamate on depolarization of rat brain slices.

Détails

ID Serval
serval:BIB_B3AB7A545059
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Release of N-acetylaspartylglutamate on depolarization of rat brain slices.
Périodique
Journal of Neurochemistry
Auteur(s)
Zollinger M., Amsler U., Do K.Q., Streit P., Cuénod M.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Statut éditorial
Publié
Date de publication
1988
Peer-reviewed
Oui
Volume
51
Numéro
6
Pages
1919-1923
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
In a great number of investigations, evidence in favor of a neurotransmitter role of the N-terminal-blocked, acidic dipeptide N-acetylaspartylglutamate (NAAG) has been accumulating. In fact, in some systems of the mammalian brain, almost all of the classical criteria for neurotransmitters have been fulfilled by NAAG except for the demonstration of its release from nervous tissue on depolarization. For quantification of NAAG in superfusates of brain slices, we have developed an analytical procedure consisting of an ion exchange prepurification, followed by a derivatization procedure and gas chromatography-mass spectrometry with chemical ionization and selected ion monitoring. Deuterated NAAG was used as an internal standard to provide a high degree of reliability for the analytical method. Detection limits of less than 1 pmol were achieved. A statistically highly significant increase of NAAG concentration in superfusates from rat neocortex, piriform cortex/amygdala, and hippocampus on depolarization with 50 mM K+ could be demonstrated and was shown to be largely Ca2+ dependent. These results support the hypothesis that NAAG is a neurotransmitter. Especially with respect to the piriform cortex, the present demonstration of NAAG release is consistent with electrophysiological and immunohistochemical evidence for its neurotransmitter function at terminals of the lateral olfactory tract.
Mots-clé
Amygdala/metabolism, Animals, Brain/drug effects, Brain/physiology, Calcium/pharmacology, Cerebral Cortex/metabolism, Chromatography, Ion Exchange, Dipeptides/metabolism, Electrophysiology, Gas Chromatography-Mass Spectrometry, Hippocampus/metabolism, Immunohistochemistry, Male, Potassium/pharmacology, Rats
Pubmed
Web of science
Création de la notice
06/03/2014 17:25
Dernière modification de la notice
20/08/2019 16:22
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