A positioned nucleosome on the human U6 promoter allows recruitment of SNAPc by the Oct-1 POU domain.

Détails

ID Serval
serval:BIB_B38458F705F6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A positioned nucleosome on the human U6 promoter allows recruitment of SNAPc by the Oct-1 POU domain.
Périodique
Molecular Cell
Auteur(s)
Zhao X., Pendergrast P.S., Hernandez N.
ISSN
1097-2765[print], 1097-2765[linking]
Statut éditorial
Publié
Date de publication
03/2001
Volume
7
Numéro
3
Pages
539-549
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human snRNA promoters contain a proximal sequence element (PSE) required for basal transcription and a distal sequence element (DSE) required for activated transcription. The PSE recruits the multisubunit factor SNAPc, whereas the DSE recruits Oct-1. Oct-1 and SNAPc bind cooperatively to DNA when their respective binding sites are moved into proximity through a mechanism that involves a defined protein-protein contact. Here, we show that on the natural U6 promoter, cooperative binding of Oct-1 and SNAPc is mediated by a positioned nucleosome that resides between the DSE and the PSE. This cooperative binding requires the same protein-protein contact as cooperative binding to closely spaced sites on naked DNA and mediates transcription activation.
Mots-clé
Allosteric Site, DNA/chemistry, DNA/genetics, DNA Footprinting, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/metabolism, Hela Cells, Host Cell Factor C1, Humans, Micrococcal Nuclease/metabolism, Models, Biological, Molecular Conformation, Nucleosomes/chemistry, Nucleosomes/genetics, Octamer Transcription Factor-1, Promoter Regions, Genetic/genetics, Protein Binding, Protein Structure, Tertiary, RNA, Messenger/genetics, RNA, Messenger/metabolism, RNA, Small Nuclear/genetics, Response Elements/genetics, Templates, Genetic, Transcription Factors/chemistry, Transcription Factors/metabolism, Transcriptional Activation
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 16:22
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