Article: article from journal or magazin.
Islet-brain1/JNK-interacting protein-1 is required for early embryogenesis in mice.
Journal of Biological Chemistry
Islet-brain1/JNK-interacting protein-1 (IB1/JIP-1) is a scaffold protein that organizes the JNK, MKK7, and MLK1 to allow signaling specificity. Targeted disruption of the gene MAPK8IP1 encoding IB1/JIP-1 in mice led to embryonic death prior to blastocyst implantation. In culture, no IB1/JIP-1(-/-) embryos were identified indicating that accelerated cell death occurred during the first cell cycles. IB1/JIP-1 expression was detected in unfertilized oocytes, in spermatozoa, and in different stages of embryo development. Thus, despite the maternal and paternal transmission of the IB1/JIP-1 protein, early transcription of the MAPK8IP1 gene is required for the survival of the fertilized oocytes.
Adaptor Proteins, Signal Transducing, Animals, Apoptosis, Blastocyst/metabolism, Blotting, Western, Carrier Proteins/physiology, Cell Death, Cell Division, Cell Survival, DNA, Complementary/metabolism, Embryo, Mammalian/metabolism, Female, Heterozygote, MAP Kinase Kinase 7, MAP Kinase Kinase Kinases/metabolism, Male, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, Mitogen-Activated Protein Kinase 8, Mitogen-Activated Protein Kinase Kinases/metabolism, Mitogen-Activated Protein Kinases/metabolism, Nuclear Proteins/physiology, Rats, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Spermatozoa/metabolism, Testis/metabolism, Trans-Activators/physiology, Transfection, Zygote/metabolism
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