The activity of S.pombe DSC-1-like factor is cell cycle regulated and dependent on the activity of p34cdc2.

Détails

ID Serval
serval:BIB_B356D55E8B5F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The activity of S.pombe DSC-1-like factor is cell cycle regulated and dependent on the activity of p34cdc2.
Périodique
Embo Journal
Auteur(s)
Reymond A., Marks J., Simanis V.
ISSN
0261-4189 (Print)
ISSN-L
0261-4189
Statut éditorial
Publié
Date de publication
1993
Volume
12
Numéro
11
Pages
4325-4334
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In the eukaryotic cell cycle, there are major control points in late G2 to determine the timing of the initiation of mitosis, and in late G1, regulating entry into S phase. In yeasts, this latter control is called start. Traverse of the start control and progression to S phase is accompanied by an increase in the expression of some of the genes whose products are required for DNA synthesis. In Saccharomyces cerevisiae, the coordinate expression of these genes in late G1 is dependent on a cis-acting sequence element called the MluI cell cycle box (MCB). A transcription factor called DSC-1 binds these elements and mediates cell cycle regulated transcription, though it is unclear whether this is by cell cycle-dependent changes in its activity. A DSC-1-like factor has also been identified in the fission yeast S.pombe. This is composed of at least the products of the cdc10 and sct1/res1 genes, and binds to the promoters of genes whose expression increases prior to S phase. We demonstrate that p85cdc10 is a nuclear protein and that the activity of the S.pombe DSC-1 factor varies through the cell cycle; it is high in cells that have passed start, decreases at the time of anaphase, remains low during the pre-start phase of G1 and increases at the time of the next S phase. We also show that the reactivation in late G1 is dependent on the G1 form of p34cdc2.
Mots-clé
Anaphase/physiology, CDC2 Protein Kinase/metabolism, Cell Cycle/physiology, Cell Cycle Proteins, Fluorescent Antibody Technique, Fungal Proteins/isolation & purification, Fungal Proteins/metabolism, G1 Phase/physiology, GTP Phosphohydrolases, Gene Expression Regulation, Fungal, Membrane Proteins, Models, Biological, Mutation, Nuclear Proteins/isolation & purification, Nuclear Proteins/metabolism, S Phase/physiology, Saccharomyces cerevisiae Proteins, Schizosaccharomyces/genetics, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 16:52
Dernière modification de la notice
03/03/2018 20:39
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