Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.

Détails

ID Serval
serval:BIB_B34B61DD77E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.
Périodique
Transgenic Research
Auteur(s)
Li W.X., Zhao H.J., Pang W.Q., Cui H.R., Poirier Y., Shu Q.Y.
ISSN
1573-9368 (Electronic)
ISSN-L
0962-8819
Statut éditorial
Publié
Date de publication
2014
Volume
23
Numéro
4
Pages
585-599
Langue
anglais
Résumé
Phytic acid (PA) is poorly digested by humans and monogastric animals and negatively affects human/animal nutrition and the environment. Rice mutants with reduced PA content have been developed but are often associated with reduced seed weight and viability, lacking breeding value. In the present study, a new approach was explored to reduce seed PA while attaining competitive yield. The OsMRP5 gene, of which mutations are known to reduce seed PA as well as seed yield and viability, was down-regulated specifically in rice seeds by using an artificial microRNA driven by the rice seed specific promoter Ole18. Seed PA contents were reduced by 35.8-71.9% in brown rice grains of transgenic plants compared to their respective null plants (non-transgenic plants derived from the same event). No consistent significant differences of plant height or number of tillers per plant were observed, but significantly lower seed weights (up to 17.8% reduction) were detected in all transgenic lines compared to null plants, accompanied by reductions of seed germination and seedling emergence. It was observed that the silencing of the OsMRP5 gene increased the inorganic P (Pi) levels (up to 7.5 times) in amounts more than the reduction of PA-P in brown rice. This indicates a reduction in P content in other cellular compounds, such as lipids and nucleic acids, which may affect overall seed development. Put together, the present study demonstrated that seed specific silencing of OsMRP5 could significantly reduce the PA content and increase Pi levels in seeds; however, it also significantly lowers seed weight in rice. Discussions were made regarding future directions towards producing agronomically competitive and nutritionally valuable low PA rice.
Mots-clé
Artificial microRNA, Seed specific promoter Ole18, Low phytic acid (LPA), Seed viability, OsMRP5, Oryza sativa L.
Pubmed
Web of science
Création de la notice
21/08/2014 8:57
Dernière modification de la notice
20/08/2019 15:21
Données d'usage