Generation of cells committed towards the photoreceptor fate for retinal transplantation

Détails

ID Serval
serval:BIB_B2F8568E9554
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Generation of cells committed towards the photoreceptor fate for retinal transplantation
Périodique
Neuroreport
Auteur(s)
Canola  K., Arsenijevic  Y.
ISSN
0959-4965 (Print)
Statut éditorial
Publié
Date de publication
06/2007
Volume
18
Numéro
9
Pages
851-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 11
Résumé
Cell transplantation is an active field of research to replace lost cells in retinal dystrophies to potentially restore visual function. We hypothesized that in-vitro differentiated retinal stem cells would integrate the appropriate retinal layer and differentiate into photoreceptors when transplanted during development. Here we show that retinal stem cells driven to the photoreceptor fate start to incorporate the retina and express photoreceptor markers but do not survive. Nevertheless surviving grafted cells express the glial marker glial fibrillary acidic protein and incorporate the ganglion cell layer as well as the inner plexiform layer. These results suggest that the maturation state of the photoreceptors is primordial to obtain robust incorporation and that a fine tuning of retinal stem cells differentiation should provide adequate cells for transplantation.
Mots-clé
3' Untranslated Regions Animals Cell Differentiation/physiology Glial Fibrillary Acidic Protein/physiology Immunohistochemistry Mice Mice, Inbred C57BL Mice, Knockout Neuroglia/physiology Photoreceptors, Vertebrate/*cytology Retina/*physiology/*transplantation Retinal Ganglion Cells/physiology/transplantation Rhodopsin/genetics/physiology Stem Cells/*physiology
Pubmed
Web of science
Création de la notice
28/01/2008 13:31
Dernière modification de la notice
03/03/2018 20:39
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